Molecular mechanism of ovarian toxicity of Hook.F. a study based on network pharmacology and molecular docking / 浙江大学学报·医学版

To explore the mechanism of ovarian toxicity of Hook. F. (TwHF) by network pharmacology and molecular docking. The candidate toxic compounds and targets of TwHF were collected by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and the Comparative Toxicogenomics Database (CTD). Then, the potential ovarian toxic targets were obtained from CTD, and the target genes of ovarian toxicity of TwHF were analyzed using the STRING database. The protein-protein interaction (PPI) network was established by Cytoscape and analyzed by the cytoHubba plug-in to identify hub genes. Additionally, the target genes of ovarian toxicity of TwHF were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses by using the R software. Finally, Discovery Studio software was used for molecular docking verification of the core toxic compounds and the hub genes. Nine candidate toxic compounds of TwHF and 56 potential ovarian toxic targets were identified in this study. Further network analysis showed that the core ovarian toxic compounds of TwHF were triptolide, kaempferol and tripterine, and the hub ovarian toxic genes included , , , , , , , , and . Besides, the GO and KEGG analysis indicated that TwHF caused ovarian toxicity through oxidative stress, reproductive system development and function, regulation of cell cycle, response to endogenous hormones and exogenous stimuli, apoptosis regulation and aging. The docking studies suggested that 3 core ovarian toxic compounds of TwHF were able to fit in the binding pocket of the 10 hub genes. TwHF may cause ovarian toxicity by acting on 10 hub genes and 140 signaling pathways.

A Neuronal Pathway that Commands Deceleration in Drosophila Larval Light-Avoidance / 神经科学通报·英文版

When facing a sudden danger or aversive condition while engaged in on-going forward motion, animals transiently slow down and make a turn to escape. The neural mechanisms underlying stimulation-induced deceleration in avoidance behavior are largely unknown. Here, we report that in Drosophila larvae, light-induced deceleration was commanded by a continuous neural pathway that included prothoracicotropic hormone neurons, eclosion hormone neurons, and tyrosine decarboxylase 2 motor neurons (the PET pathway). Inhibiting neurons in the PET pathway led to defects in light-avoidance due to insufficient deceleration and head casting. On the other hand, activation of PET pathway neurons specifically caused immediate deceleration in larval locomotion. Our findings reveal a neural substrate for the emergent deceleration response and provide a new understanding of the relationship between behavioral modules in animal avoidance responses.

Repeated Failure in Reward Pursuit Alters Innate Drosophila Larval Behaviors / 神经科学通报·英文版

Animals always seek rewards and the related neural basis has been well studied. However, what happens when animals fail to get a reward is largely unknown, although this is commonly seen in behaviors such as predation. Here, we set up a behavioral model of repeated failure in reward pursuit (RFRP) in Drosophila larvae. In this model, the larvae were repeatedly prevented from reaching attractants such as yeast and butyl acetate, before finally abandoning further attempts. After giving up, they usually showed a decreased locomotor speed and impaired performance in light avoidance and sugar preference, which were named as phenotypes of RFRP states. In larvae that had developed RFRP phenotypes, the octopamine concentration was greatly elevated, while tβh mutants devoid of octopamine were less likely to develop RFRP phenotypes, and octopamine feeding efficiently restored such defects. By down-regulating tβh in different groups of neurons and imaging neuronal activity, neurons that regulated the development of RFRP states and the behavioral exhibition of RFRP phenotypes were mapped to a small subgroup of non-glutamatergic and glutamatergic octopaminergic neurons in the central larval brain. Our results establish a model for investigating the effect of depriving an expected reward in Drosophila and provide a simplified framework for the associated neural basis.

Correlation between parental rearing patterns and self-consciousness of adolescents with cleft lip and palate / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aimed to investigate the current situation of parental rearing patterns and self-consciousness of adolescents with cleft lip and palate. The relationship between parental rearing patterns and self-consciousness of adolescents was also analyzed to provide evidence for psychological intervention.</p><p><b>METHODS</b>The study was carried out on 400 adolescents (11-25 years old) with cleft lip and palate. The subjects were assessed with self-consciousness scale for patients with cleft lip and palate and Egna Minnen av Barndoms Uppforstran family rearing questionnaires to evaluate the current status of self-consciousness and parental rearing patterns in adolescents with cleft lip and palate and the relevance of the two factors.</p><p><b>RESULTS</b>A total of 380 valid questionnaires were obtained. A significant relationship was found between parental rearing patterns and self-consciousness in adolescents with cleft lip and palate. A positive correlation was also observed between the scores of self-evaluation, self-confidence, others' evaluation and psychological endurance, interpersonal relationship, and parent-child relationship of adolescents with cleft lip and palate and the scores of their parents' emotional care. Confidence was negatively correlated with the scores of parents' refusal to deny, punishment, and severity.</p><p><b>CONCLUSIONS</b>Good parental rearing patterns play a critical role in establishing self-confidence, improving social adaptability, and maintaining interpersonal and parent-child relationship. Parents should give much care while avoiding refusal, denial, and punishment.</p>

Application of a new kind of dressing in an infectious wound care after cheiloplaty:a case report / 中国实用护理杂志

Objective To research the application of a new kind of dressing in infectious wounds care after cheiloplasty. Methods Moist wound healing therapy was applied in treating one patient with wound infection after cheiloplasty, different novel dressings were selected and the operating mode of integrated medical care was adopted for treatment. Results The wound was healing by second intention after treatment, and such treatment was associated with comfortable patient experience during dressing change, short treatment time, mild scar, excellent overall therapeutic effects, high patient and family member satisfaction. Conclusions The application of novel dressing has provided a new treatment scheme for clinical treatment of wound infection after cheiloplasty.

Application of social work program in Cleft Lip and Palate department / 中国实用护理杂志

Launching social work program in Cleft Lip and Palate department, volunteers can help provide supportive services for patients, to enhance our service level, and to benefit patients suffering and health care quality in hospital. Also this program helps develop volunteers′ skills by doing face to face practice and getting feedback from patients directly and consistently. Most importantly, patients will receive excellent services, which assist to Improve the reputation of the hospital. This article introduces how volunteers perform supportive services and also provides a specific way that volunteers, participate in Cleft Lip and Palate department

Study on postoperative infusion management of cleft patients / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The study aims to investigate the correlation between total input for a period of 24 h and the daily physiological requirements to develop practical guidelines for postoperative infusion management of cleft patients.</p><p><b>METHODS</b>Sixty-three cleft lip and palate patients under three years old who underwent surgery were included in the study. The amount of liquid taken orally as well as intravenous input were recorded for a period of 24 h and compared with the daily physiological requirements. Based on the comparison results, the amount of intravenous infusion was adjusted to match the physiological requirements. Eighty-one patients under one year old were also included to evaluate the effectiveness of the adjustment. The amount of liquid taken orally and intravenous inputs were also recorded and the correlation between total input and urine output analyzed.</p><p><b>RESULTS</b>The total input of the first group of patients significantly exceeded the physiological requirements. After adjustment, the liquid intake remained at the same level, but the total intake moved closer to the physiological requirements. Statistically significant correlations were observed between liquid taken orally and intravenous intakes, and between total intake and urine formation.</p><p><b>CONCLUSIONS</b>More precise management of postoperative infusion is necessary for cleft patients. For patients less than six months old, the amount of intravenous infusion should be around 60% of the physiological requirements, whereas that for patients between six months and one year should be around 75%.</p>

Effects of hypnotic and musical relaxation therapy on the treatment of the parents of children with cleft lip and/or palate / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the effectiveness of hypnotic and musical relaxation therapy and psychological consultation for parents of children with cleft lip and/or palate (CLP) and to provide a scientific basis of clinical-psychological treatment options.</p><p><b>METHODS</b>Sixty-six subjects with children with CLP participated in this study. The subjects were randomly assigned to a test group (n = 33) and a control group (n = 33). The test group was treated with hypnotic and musical relaxation therapy; the control group were subjected to psychological consultation. Anxiety and depression states were evaluated by using a self-rating anxiety scale (SAS) and a self-rating depression scale (SDS) before and after treatment was administered.</p><p><b>RESULTS</b>1) The test group demonstrated a significant decrease in SAS and SDS scores (t = 2.855, P < 0.01; t = 2.777, P < 0.01). The control group showed a significant decrease in the SAS score (t = 1.831, P < 0.05) but failed to show a significant change in the depression score (t = 0.909, P > 0.05). 2) The test group yielded a higher percentage of remission indicated by the SDS scores than the control group (test group = 75.76%; control group = 60.61%; P < 0.05). The test group also displayed a higher percentage of remission indicated by the SAS scores than the control group (test group = 78.79%, test group = 69.70%; P < 0.05).</p><p><b>CONCLUSION</b>Hypnotic and musical relaxation therapy can more effectively reduce the scores of the anxiety and depression states of the parents of patients with cleft lip and/or palate than psychological consultation.</p>

Analysis of quality of life of 115 parents with cleft lip and/or palate children / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the quality of life of cleft lip and/or palate children's parents and discuss the factors to provide the oretical basis for improving the quality of life of these parents and promoting the healthy growth of children with cleft lip and/or palate.</p><p><b>METHODS</b>A total of 115 parents whose children had cleft lip and/or palate surgery treatment were selected as the experiment group, and another 198 parents (with healthy children having a similar age with those in the experiment group) as the control group. The experiment group was divided into three subgroups according to different types of cleft lip and/or palate: cleft Lip (CL), cleft palate (CP), cleft lip and palate (CLP). The experiment group and the control group were both divided into four subgroups according to age: 0-1, 1-3, 3-6 years old, and more than 6 years old. The experiment group and the control group were both divided into three subgroups according to education: junior middle school and the following, high school and technical secondary school, junior college degree or above. The GQOLI-74 scale was selected to assess the experiment group and the control group. SPSS 16.0 software was used to analyze data.</p><p><b>RESULTS</b>1) The experiment group had no significant difference with the control group in terms of the overall score and the scores of various children ages. 2) The scores of every item had no significant difference in CL, CP, CLP subgroup (P > 0.05). 3) The quality of life scores and scores of psychological function dimension and social function dimension of parents with 3-6 years old patients were obviously lower than those of parents with more than 6 years old patients (P<0.05). The scores of social function dimension of parents with 0-1, 1-3, 3-6 years old patients were obviously lower than those of parents with more than 6 years old patients (P < 0.05). The other items had no significant difference. 4) The scores of material life dimension and social function dimension of parents with junior college degree or above were higher than those of parents with junior middle school degree and the following (P < 0.05). The scores of social function dimension of parents with high school and technical secondary school degree were higher than those of parents with junior middle school degree and the following (P < 0.05).</p><p><b>CONCLUSION</b>No difference was observed in the quality of life between cleft lip and/or palate children's parents and normal group. The parents with the low age children with cleft lip and/or palate and low-levels of education need more help and support to improve quality of life.</p>

Nursing intervention and evaluation of postoperative pain in preschool children with cleft lip and palate / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This study aimed to observe the postoperative pain rate and degree of pain in preschool children with cleft lip and palate, and investigate the effect of nursing intervention on pain relief.</p><p><b>METHODS</b>A total of 120 hospitalized cases of three- to seven-year-old preschool children with cleft lip and palate were selected from May to October 2011. The subjects were randomly divided into the control group and experimental groups 1, 2, and 3. The control group used conventional nursing methods, experimental group 1 used analgesic drug treatment, experimental group 2 used psychological nursing interventions, and experimental group 3 used both psychological nursing intervention and analgesic drug treatment. After 6, 12, 24, and 48 h, pain self-assessment, pain parent-assessment, and pain nurse-assessment were calculated for the four groups using the pain assessment forms, and their ratings were compared.</p><p><b>RESULTS</b>The postoperative pain rates of the four groups ranged from 50.0% to 73.3%. The difference among the four groups was statistically significant (P < 0.001). The differences among the control group and experimental groups 1 and 2 were not statistically significant (P = 0.871), whereas the differences among experimental group 3 and the other groups were statistically significant (P < 0.001).</p><p><b>CONCLUSION</b>Postoperative pain in preschool children with cleft lip and palate is common. Psychological nursing intervention with analgesic treatment is effective in relieving postoperative pain.</p>

Effect of Tripterygium wilfordii on gonadal injury and progression of its relative Chinese medicine therapy / 中国中药杂志

The paper reviewed the clinical manifestation and potential mechanism of gonadal injury of Tripterygium wilfordii and the progression of its relative Chinese medicine therapy. The therapeutic effect of T. wilfordii is certain, but its adverse effect is obvious. Therefore, it is very important to investigate the relative therapy for the exploration of rationality and regularity of drug compatibility.

Toxicity-reducing and Action-enhancing Effects of Total Glucosides of Paeony on Tripterygium Glycosides in Treating Lupus Nephritis / 中药新药与临床药理

Objective To investigate the toxicity-reducing and action-enhancing effects of total glucosides of paeony (TGP) on tripterygium glycosides (TG) in treating murine lupus nephritis (LN).Methods Chronic graft-versus-host disease (cGVHD) murine LN model was established.Modeling mice were randomly divided into three groups:LN group,TG group (in the dose of 30 mg?kg-1?d-1),and TG+TGP group (TG in the dose of 30 mg?kg-1?d-1 and TGP in the dose of 200 mg?kg-1?d-1),6 mice in each group.Another six normal mice served as control.Medication groups received corresponding medicine according to the experimental design,and mice in the normal control group and LN group received the same volume of saline,qd,for 4 weeks.After the treatment,the levels of 24-hour urinary protein excretion (UPE),blood urea nitrogen (BUN),serum creatinine (SCr),serum alanine transferase (ALT) and aspartate transferase (AST),and contents of superoxide dismustase (SOD),malondialdehyde (MDA),and glutathione peroxidase (GSH-Px) in liver tissue homogenate were measured.Meanwhile,the pathologic changes of kidneys in each group were detected.Results Compared with LN group,levels of UPE,BUN and SCr in TG group and TG+TGP group were decreased notablely (P